Selective immunocapture and light-controlled traceless release of transiently caged proteins

Summary: The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-c...

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Bibliographic Details
Main Authors: Rasa Rakauskaitė, Giedrė Urbanavičiūtė, Martynas Simanavičius, Aurelija Žvirblienė, Saulius Klimašauskas
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001623