Computational design and characterization of a temperature-sensitive plasmid replicon for gram positive thermophiles

<p><b>Abstract</b></p> <p><b>Background</b></p> <p>Temperature-sensitive (Ts) plasmids are useful tools for genetic engineering, but there are currently none compatible with the gram positive, thermophilic, obligate anaerobe, <it>Clostridiu...

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Bibliographic Details
Main Authors: Olson Daniel G, Lynd Lee R
Format: Article
Language:English
Published: BMC 2012-05-01
Series:Journal of Biological Engineering
Subjects:
Online Access:http://www.jbioleng.org/content/6/1/5