High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity

High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity

Reduced costs of sequencing have tremendously impacted the field of microbial ecology, allowing scientists to design more studies with larger sample sizes that often exceed 10,000 samples. Library preparation costs have not kept pace with sequencing prices, although automated liquid handling robots...

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Bibliographic Details
Main Authors: Jeremiah J. Minich, Greg Humphrey, Rodolfo A. S. Benitez, Jon Sanders, Austin Swafford, Eric E. Allen, Rob Knight
Format: Article
Language:English
Published: American Society for Microbiology 2018-11-01
Series:mSystems
Subjects:
DNA metabarcoding
Illumina MiSeq
NGS
acoustic liquid handler
automation
library preparation
metabarcoding
microbial ecology
microbiome
Online Access:https://doi.org/10.1128/mSystems.00166-18

Internet

https://doi.org/10.1128/mSystems.00166-18

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