A new method for extracting DNA from the grape berry surface, beginning in the vineyard
Isolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface...
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Elsevier
2019-10-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019362577 |
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doaj-b3e4b7231a304d3994636b55d5b0d9572020-11-25T02:07:06ZengElsevierHeliyon2405-84402019-10-01510e02597A new method for extracting DNA from the grape berry surface, beginning in the vineyardMegan E. Hall0Lance Cadle-Davidson1Zhiwei Fang2Wayne F. Wilcox3Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, NYS Agricultural Experiment Station, Geneva, NY, 14456, United States; Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, United States; Corresponding author.United States Department of Agriculture–Agricultural Research Service, Grape Genetics Research Unit, Geneva, NY, 14456, United StatesDivision of Plant Sciences, University of Missouri, Columbia, MO, 65211, United StatesSection of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, Cornell University, NYS Agricultural Experiment Station, Geneva, NY, 14456, United StatesIsolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface while en route to the lab through agitation, and efficiently extracts microbial DNA from the surface of the grape. It is cost effective and utilizes commonly available laboratory chemicals with low toxicity (Table 1). This protocol allows researchers to extract DNA from the grape berry surface in the field, therefore undergoing minimal manipulation of those microbiomes before DNA extraction.http://www.sciencedirect.com/science/article/pii/S2405844019362577Agricultural scienceMicrobiologyPlant biologyVineyardDNA extractionField |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Megan E. Hall Lance Cadle-Davidson Zhiwei Fang Wayne F. Wilcox |
| spellingShingle |
Megan E. Hall Lance Cadle-Davidson Zhiwei Fang Wayne F. Wilcox A new method for extracting DNA from the grape berry surface, beginning in the vineyard Heliyon Agricultural science Microbiology Plant biology Vineyard DNA extraction Field |
| author_facet |
Megan E. Hall Lance Cadle-Davidson Zhiwei Fang Wayne F. Wilcox |
| author_sort |
Megan E. Hall |
| title |
A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
| title_short |
A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
| title_full |
A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
| title_fullStr |
A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
| title_full_unstemmed |
A new method for extracting DNA from the grape berry surface, beginning in the vineyard |
| title_sort |
new method for extracting dna from the grape berry surface, beginning in the vineyard |
| publisher |
Elsevier |
| series |
Heliyon |
| issn |
2405-8440 |
| publishDate |
2019-10-01 |
| description |
Isolating DNA from microbes on the surface of a grape berry is a challenge due to their adhesion to the thick berry skin and cuticle, making studies of the grape microbiome challenging. We developed a field-to-lab DNA extraction procedure that starts in the vineyard, disrupts the grape berry surface while en route to the lab through agitation, and efficiently extracts microbial DNA from the surface of the grape. It is cost effective and utilizes commonly available laboratory chemicals with low toxicity (Table 1). This protocol allows researchers to extract DNA from the grape berry surface in the field, therefore undergoing minimal manipulation of those microbiomes before DNA extraction. |
| topic |
Agricultural science Microbiology Plant biology Vineyard DNA extraction Field |
| url |
http://www.sciencedirect.com/science/article/pii/S2405844019362577 |
| work_keys_str_mv |
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