Metformin combined with cisplatin inhibits the ovarian cancer cell growth and invasion in vitro: an experimental study
Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM, the DDP group...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Editorial Board of Journal of Hainan Medical University
2018-01-01
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Series: | Journal of Hainan Medical University |
Subjects: | |
Online Access: | http://www.hnykdxxb.com/PDF/201801/02.pdf |
Summary: | Objective: To study the effect of metformin combined with cisplatin on the expression of
genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian
cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM, the DDP group treated with serum-free DMEM containing 10 μmol/L cisplatin,
the MET group treated with serum-free DMEM containing 10 mmol/L metformin and the
DDP+MET group treated with serum-free DMEM containing 10 μmol/L cisplatin and 10
mmol/L metformin. Results: 24 h after treatment, GRP78, TBX2, CyclinD1, Ki-67, MTA1,
CTHRC1, Slug and MMP2 protein expression in DDP group, MET group and DDP+MET
group were significantly lower than those in control group whereas Fn14, NDRG2, SOCS1,
Beclin1 and ST7L protein expression were significantly higher than those of control group;
GRP78, TBX2, CyclinD1, Ki-67, MTA1, CTHRC1, Slug and MMP2 protein expression in
DDP+MET group were significantly lower than those in DDP group and MET group whereas
Fn14, NDRG2, SOCS1, Beclin1 and ST7L protein expression were significantly higher than
those in DDP group and MET group. Conclusion: metformin combined with cisplatin can
inhibit the expression of pro-proliferation and invasion genes and promote the expression of
pro-apoptosis genes in ovarian cancer cells so as to inhibit cancer cell growth and invasion. |
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ISSN: | 1007-1237 1007-1237 |