Metformin combined with cisplatin inhibits the ovarian cancer cell growth and invasion in vitro: an experimental study

Metformin combined with cisplatin inhibits the ovarian cancer cell growth and invasion in vitro: an experimental study

Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM, the DDP group...

Full description

Bibliographic Details
Main Authors: Ni Liu, Dan Pi, Chun-Mei Liu
Format: Article
Language:English
Published: Editorial Board of Journal of Hainan Medical University 2018-01-01
Series:Journal of Hainan Medical University
Subjects:
Department of Obstetrics and Gynecology
Chongqing Qianjiang National Hospital
Chongqing
409000. Fund Project: Science and Technology Project of Qianjiang District No: 2016056. 1. Introduction Ovarian cancer is one of the most common malignant tumors in the female reproductive system. Surgical resection and chemotherapy are the main treatments. Because ovarian cancer cells are more sensitive to chemotherapy drugs
especially platinum drugs
the platinum-based intravenous chemotherapy is widely used in the treatment of advanced ovarian cancer[1
some patients with ovarian cancer can develop drug resistance during chemotherapy
which will affect the efficacy of chemotherapy[3]. Metformin is a kind of insulin sensitizer for diabetes treatment
it has been confirmed in recent years that it has anti-tumor effect and chemotherapy drug sensitization effect
and its combination with a variety of chemotherapy drugs can enhance the killing effect of chemotherapy drugs on tumor cells[4]. In the progression of the course of ovarian cancer
the growth and invasion of cancer cells involve the changes in the expression of multiple proliferationrelated genes and invasion-related genes. In the following studies
we specifically analyzed the effects of metformin combined with cisplatin on the expression of genes associated with ovarian cancer cell growth and invasion in vitro. 2. Experimental materials and methods 2.1 Experimental materials Ovarian cancer SKOV3 cell lines were bought in the cell bank of Chinese Academy of Sciences
DMEM
fetal bovine serum and 0.125% trypsin for cell culture were purchased in Hyclone Company
cisplatin and metformin were bought in the Sigma Company
RIPA lysate was purchased in Shanghai Beyotime Company
and enzyme-linked immunosorbent assay kits were purchased from Shanghai Westang Biotechnology Company. Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM
the DDP group treated with serum-free DMEM containing 10 μmol/L cisplatin
the MET group treated with serum-free DMEM containing 10 mmol/L metformin and the DDP+MET group treated with serum-free DMEM containing 10 μmol/L cisplatin and 10 mmol/L metformin. Results: 24 h after treatment
GRP78
TBX2
CyclinD1
Ki-67
Ovarian cancer
Metformin
Cisplatin
Proliferation
Invasion
Online Access:http://www.hnykdxxb.com/PDF/201801/02.pdf

Internet

http://www.hnykdxxb.com/PDF/201801/02.pdf

Similar Items