Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells

Optimized protocol for the identification of lipid droplet proteomes using proximity labeling proteomics in cultured human cells

Summary: Lipid droplets are endoplasmic reticulum-derived neutral lipid storage organelles that play critical roles in cellular lipid and energy homeostasis. Here, we present a protocol for the identification of high-confidence lipid droplet proteomes in a cell culture model. This approach overcomes...

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Bibliographic Details
Main Authors: Clark W.H. Peterson, Kirandeep K. Deol, Milton To, James A. Olzmann
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Cell Membrane
Cell separation/fractionation
Cell-based Assays
Proteomics
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002860

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http://www.sciencedirect.com/science/article/pii/S2666166721002860

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