Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities

Genome-wide libraries for CRISPR knockout, interference, and activation have allowed the systemic interrogation of gene function. Here, the authors evaluate the Brunello CRISPRko library and introduce Dolcetto and Calabrese for CRISPRi and CRISPRa, respectively.

Bibliographic Details
Main Authors: Kendall R. Sanson, Ruth E. Hanna, Mudra Hegde, Katherine F. Donovan, Christine Strand, Meagan E. Sullender, Emma W. Vaimberg, Amy Goodale, David E. Root, Federica Piccioni, John G. Doench
Format: Article
Language:English
Published: Nature Publishing Group 2018-12-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-07901-8

Internet

https://doi.org/10.1038/s41467-018-07901-8

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