Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product

<p>Abstract</p> <p>In this study, a putative esterase, designated EstMY, was isolated from an activated sludge metagenomic library. The lipolytic gene was subcloned and expressed in <it>Escherichia coli </it>BL21 using the pET expression system. The gene <it>estMY...

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Bibliographic Details
Main Authors: WenJun Han, KeGui Zhang, JunGang Li
Format: Article
Language:English
Published: BMC 2010-11-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/9/1/83