One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection

Abstract Background Zika virus (ZIKV) is transmitted to humans during the bite of an infected mosquito. In a scenario of globalization and climate change, the frequency of outbreaks has and will increase in areas with competent vectors, revealing a need for continuous improvement of ZIKV detection t...

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Bibliographic Details
Main Authors: Ricardo Vieira Araujo, Fabiana Feitosa-Suntheimer, Alexander S. Gold, Berlin Londono-Renteria, Tonya M. Colpitts
Format: Article
Language:English
Published: BMC 2020-03-01
Series:Parasites & Vectors
Subjects:
Aedes aegypti
Zika virus
One-step qRT-PCR
Mosquito gene expression
Online Access:http://link.springer.com/article/10.1186/s13071-020-4002-x

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http://link.springer.com/article/10.1186/s13071-020-4002-x

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