Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival

Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival

Vascular endothelial growth factor-C/D (VEGF-C/D) regulates lymphangiogenesis. Ingrowth of lymphatic vessels is negatively associated with corneal transplantation success. In this study, we therefore analyzed the effect local blockade of VEGF-C/D has on inflamed corneas. We used the murine model of...

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Main Authors: Ann-Charlott Salabarria, Manuel Koch, Alfrun Schönberg, Elisabeth Zinser, Deniz Hos, Matthias Hamdorf, Thomas Imhof, Gabriele Braun, Claus Cursiefen, Felix Bock
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Journal of Clinical Medicine
Subjects:
VEGF C/D trap
corneal transplantation
local immune modulation
Online Access:https://www.mdpi.com/2077-0383/9/5/1270
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spelling doaj-b1a1b10c44684b88876a8c763018a2c52020-11-25T03:05:54ZengMDPI AGJournal of Clinical Medicine2077-03832020-04-0191270127010.3390/jcm9051270Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft SurvivalAnn-Charlott Salabarria0Manuel Koch1Alfrun Schönberg2Elisabeth Zinser3Deniz Hos4Matthias Hamdorf5Thomas Imhof6Gabriele Braun7Claus Cursiefen8Felix Bock9Department of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyCenter for Molecular Medicine Cologne (CMMC), University of Cologne, 50937 Cologne, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyDepartment of Immune Modulation, Universitätsklinikum Erlangen, Friedrixch-Alexander-Universität Erlangen-Nuremberg, D-91052 Erlangen, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyCenter for Molecular Medicine Cologne (CMMC), University of Cologne, 50937 Cologne, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyDepartment of Ophthalmology, University of Cologne, Faculty of Medicine and University Hospital Cologne, 50937 Cologne, GermanyVascular endothelial growth factor-C/D (VEGF-C/D) regulates lymphangiogenesis. Ingrowth of lymphatic vessels is negatively associated with corneal transplantation success. In this study, we therefore analyzed the effect local blockade of VEGF-C/D has on inflamed corneas. We used the murine model of suture-induced neovascularization and subsequent high-risk corneal transplantation. Mice were treated with a VEGF-C/D trap prior to transplantation. Topical inhibition of VEGF-C/D significantly reduced lymphatic vessel ingrowth, but increased Macrophage numbers in the cornea. Furthermore, corneal transplantation success was not improved by the topical application of the compound. This study demonstrates that local VEGF-C/D inhibition is insufficient to increases corneal transplantation success, likely due to interaction with immune cells.https://www.mdpi.com/2077-0383/9/5/1270VEGF C/D trapcorneal transplantationlocal immune modulation
collection DOAJ
language English
format Article
sources DOAJ
author Ann-Charlott Salabarria
Manuel Koch
Alfrun Schönberg
Elisabeth Zinser
Deniz Hos
Matthias Hamdorf
Thomas Imhof
Gabriele Braun
Claus Cursiefen
Felix Bock
spellingShingle Ann-Charlott Salabarria
Manuel Koch
Alfrun Schönberg
Elisabeth Zinser
Deniz Hos
Matthias Hamdorf
Thomas Imhof
Gabriele Braun
Claus Cursiefen
Felix Bock
Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
Journal of Clinical Medicine
VEGF C/D trap
corneal transplantation
local immune modulation
author_facet Ann-Charlott Salabarria
Manuel Koch
Alfrun Schönberg
Elisabeth Zinser
Deniz Hos
Matthias Hamdorf
Thomas Imhof
Gabriele Braun
Claus Cursiefen
Felix Bock
author_sort Ann-Charlott Salabarria
title Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
title_short Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
title_full Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
title_fullStr Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
title_full_unstemmed Topical VEGF-C/D Inhibition Prevents Lymphatic Vessel Ingrowth into Cornea but Does Not Improve Corneal Graft Survival
title_sort topical vegf-c/d inhibition prevents lymphatic vessel ingrowth into cornea but does not improve corneal graft survival
publisher MDPI AG
series Journal of Clinical Medicine
issn 2077-0383
publishDate 2020-04-01
description Vascular endothelial growth factor-C/D (VEGF-C/D) regulates lymphangiogenesis. Ingrowth of lymphatic vessels is negatively associated with corneal transplantation success. In this study, we therefore analyzed the effect local blockade of VEGF-C/D has on inflamed corneas. We used the murine model of suture-induced neovascularization and subsequent high-risk corneal transplantation. Mice were treated with a VEGF-C/D trap prior to transplantation. Topical inhibition of VEGF-C/D significantly reduced lymphatic vessel ingrowth, but increased Macrophage numbers in the cornea. Furthermore, corneal transplantation success was not improved by the topical application of the compound. This study demonstrates that local VEGF-C/D inhibition is insufficient to increases corneal transplantation success, likely due to interaction with immune cells.
topic VEGF C/D trap
corneal transplantation
local immune modulation
url https://www.mdpi.com/2077-0383/9/5/1270
work_keys_str_mv AT anncharlottsalabarria topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
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AT elisabethzinser topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT denizhos topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT matthiashamdorf topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT thomasimhof topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT gabrielebraun topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT clauscursiefen topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
AT felixbock topicalvegfcdinhibitionpreventslymphaticvesselingrowthintocorneabutdoesnotimprovecornealgraftsurvival
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