Massively parallel RNA device engineering in mammalian cells with RNA-Seq

Synthetic RNA-based devices can dynamically control a wide range of processes. Here the authors develop a quantitative and high-throughput mammalian cell-based RNA-seq assay to efficiently engineer ribozyme switches.

Bibliographic Details
Main Authors: Joy S. Xiang, Matias Kaplan, Peter Dykstra, Michaela Hinks, Maureen McKeague, Christina D. Smolke
Format: Article
Language:English
Published: Nature Publishing Group 2019-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12334-y