Anti-EGFR V<sub>HH</sub> Antibody under Thermal Stress Is Better Solubilized with a Lysine than with an Arginine SEP Tag

Anti-EGFR V<sub>HH</sub> Antibody under Thermal Stress Is Better Solubilized with a Lysine than with an Arginine SEP Tag

In this study, we assessed the potential of arginine and lysine solubility-enhancing peptide (SEP) tags to control the solubility of a model protein, anti-EGFR V<sub>HH</sub>-7D12, in a thermally denatured state at a high temperature. We produced V<sub>HH</sub>-7D12 antibodie...

Full description

Bibliographic Details
Main Authors: Md. Golam Kibria, Akari Fukutani, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Yutaka Kuroda
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Biomolecules
Subjects:
SEP tag
aggregation
single domain antibody
V<sub>HH</sub>
arginine SEP tag
lysine SEP tag
Online Access:https://www.mdpi.com/2218-273X/11/6/810

Internet

https://www.mdpi.com/2218-273X/11/6/810

Similar Items