Analytic Pearson residuals for normalization of single-cell RNA-seq UMI data
Abstract Background Standard preprocessing of single-cell RNA-seq UMI data includes normalization by sequencing depth to remove this technical variability, and nonlinear transformation to stabilize the variance across genes with different expression levels. Instead, two recent papers propose to use...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-09-01
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| Series: | Genome Biology |
| Online Access: | https://doi.org/10.1186/s13059-021-02451-7 |