SYBR Green real-time PCR-RFLP assay targeting the plasmodium cytochrome B gene--a highly sensitive molecular tool for malaria parasite detection and species determination.

A prerequisite for reliable detection of low-density Plasmodium infections in malaria pre-elimination settings is the availability of ultra-sensitive and high-throughput molecular tools. We developed a SYBR Green real-time PCR restriction fragment length polymorphism assay (cytb-qPCR) targeting the...

Full description

Bibliographic Details
Main Authors: Weiping Xu, Ulrika Morris, Berit Aydin-Schmidt, Mwinyi I Msellem, Delér Shakely, Max Petzold, Anders Björkman, Andreas Mårtensson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4361616?pdf=render