Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis
α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production....
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Instituto de Tecnologia do Paraná (Tecpar)
2010-02-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026 |
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doaj-af57a283f4bd4a989bf75bcd943a8dc22020-11-24T22:56:05ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242010-02-0153121121810.1590/S1516-89132010000100026Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysisShankar KapnoorVeerappa Hanumanth Mulimaniα-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of soymilk were 55(0)C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026Aspergillus oryzaeα-galactosidaseSSFsoymilkinvertaseHPLC analysis |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shankar Kapnoor Veerappa Hanumanth Mulimani |
| spellingShingle |
Shankar Kapnoor Veerappa Hanumanth Mulimani Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis Brazilian Archives of Biology and Technology Aspergillus oryzae α-galactosidase SSF soymilk invertase HPLC analysis |
| author_facet |
Shankar Kapnoor Veerappa Hanumanth Mulimani |
| author_sort |
Shankar Kapnoor |
| title |
Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis |
| title_short |
Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis |
| title_full |
Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis |
| title_fullStr |
Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis |
| title_full_unstemmed |
Production of α-Galactosidase by Aspergillus oryzae through solid-state fermentation and its application in soymilk Galactooligosaccharide hydrolysis |
| title_sort |
production of α-galactosidase by aspergillus oryzae through solid-state fermentation and its application in soymilk galactooligosaccharide hydrolysis |
| publisher |
Instituto de Tecnologia do Paraná (Tecpar) |
| series |
Brazilian Archives of Biology and Technology |
| issn |
1516-8913 1678-4324 |
| publishDate |
2010-02-01 |
| description |
α-Galactosidase was produced by Aspergillus oryzae on red gram plant waste-wheat bran based media in solid-state fermentation (SSF). Optimum temperature for α-galactosidase production was 35 0C and upto 4 cm of bed height of substrate had no inhibitory effect on enzyme production. Hydrolysis of galactooligosaccharides in soymilk was carried out by α-galactosidase. Optimum temperature and pH for the hydrolysis of raffinose and stachyose of soymilk were 55(0)C and 5.2-6.2, respectively. The enzymatic treatment for 3 h completely removed the raffinose oligosaccharides in soymilk. Crude extract also showed considerable amount of invertase activity. |
| topic |
Aspergillus oryzae α-galactosidase SSF soymilk invertase HPLC analysis |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100026 |
| work_keys_str_mv |
AT shankarkapnoor productionof945galactosidasebyaspergillusoryzaethroughsolidstatefermentationanditsapplicationinsoymilkgalactooligosaccharidehydrolysis AT veerappahanumanthmulimani productionof945galactosidasebyaspergillusoryzaethroughsolidstatefermentationanditsapplicationinsoymilkgalactooligosaccharidehydrolysis |
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