Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.

Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.

Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentra...

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Main Authors: Dieanira Erudaitius, Andrew Huang, Sarah Kazmi, Garry R Buettner, Victor G J Rodgers
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5249139?pdf=render
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spelling doaj-af3289775f8f4a10aa80122c6f3b28cd2020-11-25T02:08:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e017044210.1371/journal.pone.0170442Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.Dieanira ErudaitiusAndrew HuangSarah KazmiGarry R BuettnerVictor G J RodgersCancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.http://europepmc.org/articles/PMC5249139?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Dieanira Erudaitius
Andrew Huang
Sarah Kazmi
Garry R Buettner
Victor G J Rodgers
spellingShingle Dieanira Erudaitius
Andrew Huang
Sarah Kazmi
Garry R Buettner
Victor G J Rodgers
Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
PLoS ONE
author_facet Dieanira Erudaitius
Andrew Huang
Sarah Kazmi
Garry R Buettner
Victor G J Rodgers
author_sort Dieanira Erudaitius
title Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
title_short Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
title_full Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
title_fullStr Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
title_full_unstemmed Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
title_sort peroxiporin expression is an important factor for cancer cell susceptibility to therapeutic h2o2: implications for pharmacological ascorbate therapy.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.
url http://europepmc.org/articles/PMC5249139?pdf=render
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AT andrewhuang peroxiporinexpressionisanimportantfactorforcancercellsusceptibilitytotherapeutich2o2implicationsforpharmacologicalascorbatetherapy
AT sarahkazmi peroxiporinexpressionisanimportantfactorforcancercellsusceptibilitytotherapeutich2o2implicationsforpharmacologicalascorbatetherapy
AT garryrbuettner peroxiporinexpressionisanimportantfactorforcancercellsusceptibilitytotherapeutich2o2implicationsforpharmacologicalascorbatetherapy
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