Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells

A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to...

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Main Authors: Lauren Gwynne, Adam C. Sedgwick, Jordan E. Gardiner, George T. Williams, Gyoungmi Kim, John P. Lowe, Jean-Yves Maillard, A. Toby A. Jenkins, Steven D. Bull, Jonathan L. Sessler, Juyoung Yoon, Tony D. James
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Chemistry
Subjects:
reaction-based fluorescent probe
alkaline phosphatase
cell imaging
fluorescence
colorimetric
Online Access:https://www.frontiersin.org/article/10.3389/fchem.2019.00255/full
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spelling doaj-ae9fce9b90ef4034b21f66ac57104b9e2020-11-25T01:14:55ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-04-01710.3389/fchem.2019.00255446203Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live CellsLauren Gwynne0Adam C. Sedgwick1Jordan E. Gardiner2George T. Williams3Gyoungmi Kim4John P. Lowe5Jean-Yves Maillard6A. Toby A. Jenkins7Steven D. Bull8Jonathan L. Sessler9Juyoung Yoon10Tony D. James11Department of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Texas at Austin, Austin, TX, United StatesDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry and Nano Science, Ewha Womans University, Seoul, South KoreaDepartment of Chemistry, University of Bath, Bath, United KingdomCardiff School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, United KingdomDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Bath, Bath, United KingdomDepartment of Chemistry, University of Texas at Austin, Austin, TX, United StatesDepartment of Chemistry and Nano Science, Ewha Womans University, Seoul, South KoreaDepartment of Chemistry, University of Bath, Bath, United KingdomA long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.https://www.frontiersin.org/article/10.3389/fchem.2019.00255/fullreaction-based fluorescent probealkaline phosphatasecell imagingfluorescencecolorimetric
collection DOAJ
language English
format Article
sources DOAJ
author Lauren Gwynne
Adam C. Sedgwick
Jordan E. Gardiner
George T. Williams
Gyoungmi Kim
John P. Lowe
Jean-Yves Maillard
A. Toby A. Jenkins
Steven D. Bull
Jonathan L. Sessler
Juyoung Yoon
Tony D. James
spellingShingle Lauren Gwynne
Adam C. Sedgwick
Jordan E. Gardiner
George T. Williams
Gyoungmi Kim
John P. Lowe
Jean-Yves Maillard
A. Toby A. Jenkins
Steven D. Bull
Jonathan L. Sessler
Juyoung Yoon
Tony D. James
Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
Frontiers in Chemistry
reaction-based fluorescent probe
alkaline phosphatase
cell imaging
fluorescence
colorimetric
author_facet Lauren Gwynne
Adam C. Sedgwick
Jordan E. Gardiner
George T. Williams
Gyoungmi Kim
John P. Lowe
Jean-Yves Maillard
A. Toby A. Jenkins
Steven D. Bull
Jonathan L. Sessler
Juyoung Yoon
Tony D. James
author_sort Lauren Gwynne
title Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_short Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_full Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_fullStr Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_full_unstemmed Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells
title_sort long wavelength tcf-based fluorescent probe for the detection of alkaline phosphatase in live cells
publisher Frontiers Media S.A.
series Frontiers in Chemistry
issn 2296-2646
publishDate 2019-04-01
description A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence “turn on” (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.
topic reaction-based fluorescent probe
alkaline phosphatase
cell imaging
fluorescence
colorimetric
url https://www.frontiersin.org/article/10.3389/fchem.2019.00255/full
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