Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes

Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes

Summary: Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Co...

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Bibliographic Details
Main Authors:	Josef Oeckl, Andrea Bast-Habersbrunner, Tobias Fromme, Martin Klingenspor, Yongguo Li
Format:	Article
Language:	English
Published:	Elsevier 2020-12-01
Series:	STAR Protocols
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166720301052

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