Isolation, Culture, and Functional Analysis of Murine Thermogenic Adipocytes
Summary: Studying brown and brite adipose tissue requires precise and reliable quantification of cellular thermogenesis. This protocol describes the isolation of primary murine pre-adipocytes, differentiation into thermogenic brown and brite adipocytes, and subsequent oxygen consumption analysis. Co...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-12-01
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Series: | STAR Protocols |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301052 |