Predictive design of sigma factor-specific promoters

Automated design tools and tailored subunits are beneficial in fine-tuning all components of a complex genetic circuit. Here the authors create E. coli and B. subtilis promoter libraries using FACS and HTS, from which an online promoter design tool has been developed using CNN.

Bibliographic Details
Main Authors: Maarten Van Brempt, Jim Clauwaert, Friederike Mey, Michiel Stock, Jo Maertens, Willem Waegeman, Marjan De Mey
Format: Article
Language:English
Published: Nature Publishing Group 2020-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19446-w