Differentiation of virulent Shimen and vaccine C strains of classical swine fever virus by duplex reverse-transcription polymerase chain reaction

It is difficult to differentiate pigs that have been injected attenuated vaccines from those that are infected by a virulent virus. In this study, a duplex reverse transcription polymerase chain reaction (RT-PCR) was developed for simultaneous detection and identification of the pathogenic strain (S...

Full description

Bibliographic Details
Main Authors: Hai-min Li, Zi-yin Zhao, Kang-kang Guo, Yan-fen Jiang, Wei-min Zhang, Yan-ming Zhang, Wei Liu, Jing-yu Wang
Format: Article
Language:English
Published: Taylor & Francis Group 2017-09-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:http://dx.doi.org/10.1080/13102818.2017.1355263