Introduction of Large Sequence Inserts by CRISPR-Cas9 To Create Pathogenicity Mutants in the Multinucleate Filamentous Pathogen Sclerotinia sclerotiorum
The necrotrophic fungal plant pathogen Sclerotinia sclerotiorum is responsible for substantial global crop losses annually resulting in localized food insecurity and loss of livelihood. Understanding the basis of this broad-host-range and aggressive pathogenicity is hampered by the quantitative natu...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
American Society for Microbiology
2018-06-01
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Series: | mBio |
Subjects: | |
Online Access: | https://doi.org/10.1128/mBio.00567-18 |