pKa Determination of a Histidine Residue in a Short Peptide Using Raman Spectroscopy

pKa Determination of a Histidine Residue in a Short Peptide Using Raman Spectroscopy

Determining the pKa of key functional groups is critical to understanding the pH-dependent behavior of biological proteins and peptide-based biomaterials. Traditionally, <sup>1</sup>H NMR spectroscopy has been used to determine the pKa of amino acids; however, for larger molecules and ag...

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Bibliographic Details
Main Authors: Brett H. Pogostin, Anders Malmendal, Casey H. Londergan, Karin S. Åkerfeldt
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Molecules
Subjects:
Raman spectroscopy
1H NMR spectroscopy
vibrational probes
histidine
peptides
proteins
isotopic labeling
deuterium replacement
acid dissociation constant
Online Access:https://www.mdpi.com/1420-3049/24/3/405

Internet

https://www.mdpi.com/1420-3049/24/3/405

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