Automatic Quantification of Immunohistochemically Stained Cell Nuclei Using Unsupervised Image Analysis

A method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions is presented. The image is transformed by a principal component transform. The resulting first component image is used to segment the objects from the background using dynamic thresholdin...

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Bibliographic Details
Main Authors: Petter Ranefall, Kenneth Wester, Ewert Bengtsson
Format: Article
Language:English
Published: Hindawi Limited 1998-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/1998/608293