Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastoris

Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastoris

κ-Carrageenase belongs to glycoside hydrolase family 16 and cleaves the β-(1→4) linkages of κ-carrageenan. In this study, genes encoding the full-length (cgkZ), Por secretion tail-truncated (cgkZΔPst) and carbohydrate binding domain-truncated (cgkZΔCBM) κ-carrageenase proteins were expressed in Pich...

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Bibliographic Details
Main Authors:	Yuan Yu, Zhemin Liu, Min Yang, Meng Chen, Zhihan Wei, Lixia Shi, Li Li, Haijin Mou
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2017-08-01
Series:	Frontiers in Microbiology
Subjects:	κ-carrageenase Pichia pastoris expression system gene truncation heterologous expression enzymatic characterization
Online Access:	http://journal.frontiersin.org/article/10.3389/fmicb.2017.01544/full

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