Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastoris

Characterization of Full-Length and Truncated Recombinant κ-Carrageenase Expressed in Pichia pastoris

κ-Carrageenase belongs to glycoside hydrolase family 16 and cleaves the β-(1→4) linkages of κ-carrageenan. In this study, genes encoding the full-length (cgkZ), Por secretion tail-truncated (cgkZΔPst) and carbohydrate binding domain-truncated (cgkZΔCBM) κ-carrageenase proteins were expressed in Pich...

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Bibliographic Details
Main Authors: Yuan Yu, Zhemin Liu, Min Yang, Meng Chen, Zhihan Wei, Lixia Shi, Li Li, Haijin Mou
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-08-01
Series:Frontiers in Microbiology
Subjects:
κ-carrageenase
Pichia pastoris expression system
gene truncation
heterologous expression
enzymatic characterization
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01544/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.01544/full

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