Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation
Abstract Background Quantitative polymerase chain reaction (qPCR) is the technique of choice for quantifying gene expression. While the technique itself is well established, approaches for the analysis of qPCR data continue to improve. Results Here we expand on the common base method to develop proc...
Main Authors: | Michael T. Ganger, Geoffrey D. Dietz, Patrick Headley, Sarah J. Ewing |
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Format: | Article |
Language: | English |
Published: |
BMC
2020-09-01
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Series: | BMC Bioinformatics |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12859-020-03696-y |
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