Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

Application of the common base method to regression and analysis of covariance (ANCOVA) in qPCR experiments and subsequent relative expression calculation

Abstract Background Quantitative polymerase chain reaction (qPCR) is the technique of choice for quantifying gene expression. While the technique itself is well established, approaches for the analysis of qPCR data continue to improve. Results Here we expand on the common base method to develop proc...

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Bibliographic Details
Main Authors: Michael T. Ganger, Geoffrey D. Dietz, Patrick Headley, Sarah J. Ewing
Format: Article
Language:English
Published: BMC 2020-09-01
Series:BMC Bioinformatics
Subjects:
Confidence intervals
Linear relationship
Lognormal
qPCR analysis
Statistics
Online Access:http://link.springer.com/article/10.1186/s12859-020-03696-y

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http://link.springer.com/article/10.1186/s12859-020-03696-y

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