Functional expression of Trametes versicolor thermotolerant laccase variant in Pichia pastoris

A mutational laccase gene MLcc1 was synthesized with modified codons, based on the codon bias of Pichia pastoris, in order to improve the thermal stability of Trametes versicolor laccase. The mutant gene was subcloned into the expression vector pGAPZαA and was transformed into P. pastoris strain X33...

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Bibliographic Details
Main Authors: Liang Huang, Yihan Liu, Xiaoguang Liu, Litong Ban, Yu Wang, Mingjie Li, Fuping Lu
Format: Article
Language:English
Published: Taylor & Francis Group 2016-03-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Online Access:http://dx.doi.org/10.1080/13102818.2015.1134278