Functional expression of Trametes versicolor thermotolerant laccase variant in Pichia pastoris
A mutational laccase gene MLcc1 was synthesized with modified codons, based on the codon bias of Pichia pastoris, in order to improve the thermal stability of Trametes versicolor laccase. The mutant gene was subcloned into the expression vector pGAPZαA and was transformed into P. pastoris strain X33...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2016-03-01
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Series: | Biotechnology & Biotechnological Equipment |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/13102818.2015.1134278 |