Protocol for Cloning Epithelial Stem Cell Variants from Human Lung

Summary: The plurality of clonogenic cells derived from human lung includes a spectrum of diverse p63+ stem cells responsible for the regeneration of normal epithelial tissue and disease-associated metaplastic lesions. Here, we report protocols for the cloning, expansion, and characterization of the...

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Bibliographic Details
Main Authors: Wei Rao, Suchan Niroula, Shan Wang, Matthew Vincent, Frank McKeon, Wa Xian
Format: Article
Language:English
Published: Elsevier 2020-09-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300502