Preparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line

Preparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line

Purpose: Transmembrane CD34 glycoprotein is the most important marker for identification, isolation and enumeration of hematopoietic stem cells (HSCs). We aimed in this study to clone the cDNA coding for human CD34 from KG1a cell line and stably express in mouse fibroblast cell line NIH-3T3. Such ar...

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Bibliographic Details
Main Authors: Farzaneh Shafaghat, Hajar Abbasi-Kenarsari, Jafar Majidi, Ali Akbar Movassaghpour, Dariush Shanehbandi, Tohid Kazemi
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2015-03-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
CD34
Cloning
Eukaryotic Expression
HSCs
KG1a
Online Access:http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/9-Kazemi.pdf

Internet

http://apb.tbzmed.ac.ir/Portals/0/Archive/Vol5-2015/9-Kazemi.pdf

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