Histochemical Analysis of the Role of Class I and Class II Clostridium Histolyticum Collagenase in the Degradation of Rat Pancreatic Extracellular Matrix for Islet Isolation

To understand why class II Clostridium histolyticum collagenase is much more effective than class I in the isolation of rat pancreatic islets, we analyzed the role of these collagenases in pancreatic tissue dissociation. Crude collagenase was purified and then fractionated into class I and II with d...

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Bibliographic Details
Main Authors: Greetje H. Vos-Scheperkeuter Ph.D., Paul T.R. Van Suylichem, Maike W.A. Vonk, Gerrit H.J. Wolters, Reinout Van Schilfgaarde
Format: Article
Language:English
Published: SAGE Publishing 1997-07-01
Series:Cell Transplantation
Online Access:https://doi.org/10.1177/096368979700600407