Combination of an enzymatic method and HPLC for the quantitation of cholesterol in cultured cells.

The study of the cellular events that lead to the foam cell formation requires the development of fast, accurate, and sensitive methods to quantify cholesterol in cultured cells. Here we describe a procedure that allows the rapid determination of free and total cholesterol in a reduced number of cel...

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Bibliographic Details
Main Authors: JA Contreras, M Castro, C Bocos, E Herrera, MA Lasunción
Format: Article
Language:English
Published: Elsevier 1992-06-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520415184