Catch me if you can: a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa

Catch me if you can: a biotinylated proteoliposome affinity assay for the investigation of assembly of the MexA-MexB-OprM efflux pump from Pseudomonas aeruginosa

Efflux pumps are membrane transporters that actively extrude various substrates, leading to multidrug resistance (MDR). In this study, we have designed a new test that allows investigating the assembly of the MexA-MexB-OprM efflux pump from the Gram negative bacteria Pseudomonas aeruginosa. The meth...

Full description

Bibliographic Details
Main Authors: VERONIQUE YVETTE eNTSOGO ENGUENE, Alice eVerchère, Gilles ePhan, Isabelle eBroutin, Martin ePicard
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-06-01
Series:Frontiers in Microbiology
Subjects:
multidrug resistance
efflux pump
membrane protein
proteoliposome
macro-molecular assembly
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00541/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00541/full

Similar Items