Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Cellulolytic bacteria have the potential to perform lignocellulose hydrolysis and fermentation simultaneously. The metabolic pathways of these bacteria, therefore, require more comprehensive and quantitative understanding. Using isotope tracer, gas chromatography-mass spectrometry, and metabolic flu...

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Bibliographic Details
Main Authors: Wei Xiong, Jonathan Lo, Katherine J. Chou, Chao Wu, Lauren Magnusson, Tao Dong, PinChing Maness
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-08-01
Series:Frontiers in Microbiology
Subjects:
13C-isotope tracer
cellulolytic bacteria
citrate synthase
glycolytic pathways
isoleucine biosynthesis
metabolic flux analysis
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.01947/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.01947/full

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