Protocol for high-throughput compound screening using flow cytometry in THP-1 cells

Summary: Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples. This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a hu...

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Bibliographic Details
Main Authors: Stephan H. Spangenberg, Reza Beheshti Zavareh, Luke L. Lairson
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721001076