Trans-active factors controlling the IL-2 gene in adult human T-cell subsets

Trans-active factors controlling the IL-2 gene in adult human T-cell subsets

IL-2 secretion in total or subsets of PHA/PMA-stimulated PBMC-derived human T-lymphocytes was monitored and found to be largely due to CD4+CD8− cells. The presence and functional state of transcription factors (TF) was assessed by protein-DNA interaction assays and functional transactivation experim...

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Main Authors: A. Mouzaki, R. H. Zubler, A. Doucet, D. Rungger
Format: Article
Language:English
Published: Hindawi Limited 1992-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/S0962935192000073
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spelling doaj-a5137bbb24db4101a414d3c52c661cdc2020-11-24T23:23:55ZengHindawi LimitedMediators of Inflammation0962-93511466-18611992-01-0111333710.1155/S0962935192000073Trans-active factors controlling the IL-2 gene in adult human T-cell subsetsA. Mouzaki0R. H. Zubler1A. Doucet2D. Rungger3Haematology, Geneva University Hospital, 25, rue Micheli-du-Crest, Geneva 4, CH-1211, SwitzerlandHaematology, Geneva University Hospital, 25, rue Micheli-du-Crest, Geneva 4, CH-1211, SwitzerlandHaematology, Geneva University Hospital, 25, rue Micheli-du-Crest, Geneva 4, CH-1211, SwitzerlandStation de Zoologie expérimentale, University of Geneva, 154, rte de Malagnou, Chêne- Bougeries, CH-1224, SwitzerlandIL-2 secretion in total or subsets of PHA/PMA-stimulated PBMC-derived human T-lymphocytes was monitored and found to be largely due to CD4+CD8− cells. The presence and functional state of transcription factors (TF) was assessed by protein-DNA interaction assays and functional transactivation experiments in the Xenopts oocyte system, modulating IL-2 transcription by injection of proteins. The results reveal that CD4+CD8− cells contain both, functional silencer in their resting, and positive TF in their activated states while the CD4+CD8− group contains only non-functional positive TF. This demonstrates that the on/off switch of IL-2 transcription is based on the same mechanism in primary T-lymphocytes of mouse spleen and in peripheral human CD4+CD8− cells.http://dx.doi.org/10.1155/S0962935192000073
collection DOAJ
language English
format Article
sources DOAJ
author A. Mouzaki
R. H. Zubler
A. Doucet
D. Rungger
spellingShingle A. Mouzaki
R. H. Zubler
A. Doucet
D. Rungger
Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
Mediators of Inflammation
author_facet A. Mouzaki
R. H. Zubler
A. Doucet
D. Rungger
author_sort A. Mouzaki
title Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
title_short Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
title_full Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
title_fullStr Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
title_full_unstemmed Trans-active factors controlling the IL-2 gene in adult human T-cell subsets
title_sort trans-active factors controlling the il-2 gene in adult human t-cell subsets
publisher Hindawi Limited
series Mediators of Inflammation
issn 0962-9351
1466-1861
publishDate 1992-01-01
description IL-2 secretion in total or subsets of PHA/PMA-stimulated PBMC-derived human T-lymphocytes was monitored and found to be largely due to CD4+CD8− cells. The presence and functional state of transcription factors (TF) was assessed by protein-DNA interaction assays and functional transactivation experiments in the Xenopts oocyte system, modulating IL-2 transcription by injection of proteins. The results reveal that CD4+CD8− cells contain both, functional silencer in their resting, and positive TF in their activated states while the CD4+CD8− group contains only non-functional positive TF. This demonstrates that the on/off switch of IL-2 transcription is based on the same mechanism in primary T-lymphocytes of mouse spleen and in peripheral human CD4+CD8− cells.
url http://dx.doi.org/10.1155/S0962935192000073
work_keys_str_mv AT amouzaki transactivefactorscontrollingtheil2geneinadulthumantcellsubsets
AT rhzubler transactivefactorscontrollingtheil2geneinadulthumantcellsubsets
AT adoucet transactivefactorscontrollingtheil2geneinadulthumantcellsubsets
AT drungger transactivefactorscontrollingtheil2geneinadulthumantcellsubsets
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