Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles

Real-Time qPCR as a Method for Detection of Antibody-Neutralized Phage Particles

The most common method for phage quantitation is the plaque assay, which relies on phage ability to infect bacteria. However, non-infective phage particles may preserve other biological properties; specifically, they may enter interactions with the immune system of animals and humans. Here, we demon...

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Bibliographic Details
Main Authors: Anna Kłopot, Adriana Zakrzewska, Dorota Lecion, Joanna M. Majewska, Marek A. Harhala, Karolina Lahutta, Zuzanna Kaźmierczak, Łukasz Łaczmański, Marlena Kłak, Krystyna Dąbrowska
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-11-01
Series:Frontiers in Microbiology
Subjects:
qPCR
plaque assay
phage
quantitation
antibody
neutralizing
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.02170/full

Internet

http://journal.frontiersin.org/article/10.3389/fmicb.2017.02170/full

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