Targeted Expansion of Tissue-Resident CD8+ T Cells to Boost Cellular Immunity in the Skin

• Main Authors: Samuel J. Hobbs, Jeffrey C. Nolz
• Format: Article
• Language: English
• Published: Elsevier 2019-12-01
• Series: Cell Reports
• Online Access: http://www.sciencedirect.com/science/article/pii/S2211124719314597

Summary: Tissue-resident memory (TRM) CD8+ T cells are positioned within environmental barrier tissues to provide a first line of defense against pathogen entry, but whether these specialized T cell populations can be readily boosted to increase protective immunity is ill defined. Here, we demonstrate that repeated activation of rare, endogenous TRM CD8+ T cells, using only topical application of antigenic peptide causes delayed-type hypersensitivity and increases the number of antigen-specific TRM CD8+ T cells, specifically in the challenged skin by ∼15-fold. Expanded TRM CD8+ T cells in the skin are derived from memory T cells recruited out of the circulation that became CD69+ tissue residents following a local antigen encounter. Notably, recruited circulating memory CD8+ T cells of a different antigen specificity could be coerced to become tissue resident using a dual-peptide challenge strategy. Expanded TRM CD8+ T cells significantly increase anti-viral protection, suggesting that this approach could be used to rapidly boost tissue-specific cellular immunity. : Tissue-resident memory (TRM) T cells provide a first line of host defense against pathogen invasion at environmental barrier tissues. Here, Hobbs and Nolz describe a mechanism to rapidly expand the number of antigen-specific TRM CD8+ T cells in the skin, using topical application of antigenic peptide to boost localized protective immunity. Keywords: tissue-resident T cells, memory T cells, CD8+ T cells, viral infection