Lactoperoxidase Immobilized onto Various Beads for Producing Natural Preservatives Solution

• Main Authors: Ahmad Nimatullah Al-Baarri, Masahiro Ogawa, Touch Visalsok, Shigeru Hayakawa
• Format: Article
• Language: Indonesian
• Published: Indonesian Food Technologists 2012-02-01
• Series: Jurnal Aplikasi Teknologi Pangan
• Subjects: Lactoperoxidase; immobilization; storage; hypothiocyanite; storage; hyphothiocyanite
• Online Access: http://jatp.ift.or.id/index.php/jatp/article/view/52/16
• ISSN: 2089-7693; 2460-5921

The natural preservative agent of hypothiocyanite (OSCN–) has been succeded to determine using immobilized LPO onto various types of beads or matrixes: SP Sepharose Fast Flow (SP-FF), SP Sepharose Big Beads (SP-BB), CNBr activated SP Sepharose (SP-CNBR). Lactoperoxidase (LPO) was obtained from bovine milk. The immobilization method was performed using ion exhange method and adsorption. Immobilized LPO onto matrixes has been stored for 7 days at 4˚C and 10˚C. Data of the production of OSCN– were collected before and after immobilized LPO storage. Data shows no remarkable effect of the production of OSCN– using immobilized LPO stored at 4˚C, however, the remarkable reduction has been detected in the production of OSCN– using immobilized LPO that was stored at 10˚C. As a conclusion, the 7 days storage does not change the capability of immobilized LPO for producing OSCN if the immobilized LPO was stored at 4˚C.