Unmasking of complements using proteinase-K in formalin fixed paraffin embedded renal biopsies

Unmasking of complements using proteinase-K in formalin fixed paraffin embedded renal biopsies

Renal biopsy interpretation requires histopathology, direct immunofluorescence (DIF) and electron microscopy. Formalin-fixed, paraffin-embedded tissue (FFPE) sent for light microscopy can be used for DIF after antigen retrieval. However, complement staining has not been satisfactory. We standardized...

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Bibliographic Details
Main Authors: R Nada, A Kumar, V G Kumar, K L Gupta, K Joshi
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:Indian Journal of Nephrology
Subjects:
Antigen retrieval
complement
direct immunofluorescence
formalin-fixed paraffin-embedded
membranoproliferative
proteinase-K
Online Access:http://www.indianjnephrol.org/article.asp?issn=0971-4065;year=2016;volume=26;issue=3;spage=182;epage=187;aulast=Nada

Internet

http://www.indianjnephrol.org/article.asp?issn=0971-4065;year=2016;volume=26;issue=3;spage=182;epage=187;aulast=Nada

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