An optimised method for the extraction of bacterial mRNA from plant roots infected with Escherichia coli O157:H7

Analysis of microbial gene expression during host colonisation provides valuable information on the nature of interaction, beneficial or pathogenic, and the adaptive processes involved. Isolation of bacterial mRNA for in planta analysis can be challenging where host nucleic acid may dominate the pr...

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Bibliographic Details
Main Authors: Ashleigh eHolmes, Louise eBirse, Robert Wilson Jackson, Nicola eHolden
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-06-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00286/full