Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization

Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization

The isolation of high‐quality RNA from the intervertebral disc and especially from the nucleus pulposus is challenging due to the low cellularity and high proteoglycan content of this tissue. In this study, we report a simple modification of the standard guanidinium thiocyanate‐phenol‐chloroform ext...

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Bibliographic Details
Main Authors: Stephanie Caprez, Ursula Menzel, Zhen Li, Sibylle Grad, Mauro Alini, Marianna Peroglio
Format: Article
Language:English
Published: Wiley 2018-06-01
Series:JOR Spine
Subjects:
gene expression
intervertebral disc
real‐time PCR
RNA isolation
Online Access:https://doi.org/10.1002/jsp2.1017

Internet

https://doi.org/10.1002/jsp2.1017

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