Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

<p>Abstract</p> <p>Background</p> <p>After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study. If real-time PCR is to be used to determine copy number and zygosity, it must be able to distinguish hem...

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Bibliographic Details
Main Authors: Baldwin Ian T, Gase Klaus, Bubner Ben
Format: Article
Language:English
Published: BMC 2004-07-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/4/14