Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-Staining

Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-Staining

Human Adipose-derived mesenchymal stem/stromal cells (hASCs) are of great interest because of their potential for therapeutic approaches. The method described here covers every single step necessary for hASCs isolation from subcutaneous abdominal adipose tissue, multicolor phenotyping by flow cytome...

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Bibliographic Details
Main Authors: Aldana D. Gojanovich, María C. Gimenez, Diego Masone, Tania M. Rodriguez, Ricardo A. Dewey, Laura R. Delgui, Diego M. Bustos, Marina Uhart
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-04-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
mesenchymal stem/stromal cells
adipose tissue
characterization
adipogenic differentiation
lipid droplets
fluorescence microscopy
Online Access:http://journal.frontiersin.org/article/10.3389/fcell.2018.00033/full

Internet

http://journal.frontiersin.org/article/10.3389/fcell.2018.00033/full

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