Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood sample...

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Main Authors:	Hannes Uchtenhagen, Cliff Rims, Gabriele Blahnik, I-Ting Chow, William W. Kwok, Jane H. Buckner, Eddie A. James
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-08-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms12614

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spelling	doaj-a2822371a2184c1581f604ee30e218952021-05-11T10:45:39ZengNature Publishing GroupNature Communications2041-17232016-08-017111210.1038/ncomms12614Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer stainingHannes Uchtenhagen0Cliff Rims1Gabriele Blahnik2I-Ting Chow3William W. Kwok4Jane H. Buckner5Eddie A. James6Benaroya Research Institute at Virginia Mason, Translational Research ProgramBenaroya Research Institute at Virginia Mason, Translational Research ProgramBenaroya Research Institute at Virginia Mason, Diabetes ProgramBenaroya Research Institute at Virginia Mason, Diabetes ProgramBenaroya Research Institute at Virginia Mason, Diabetes ProgramBenaroya Research Institute at Virginia Mason, Translational Research ProgramBenaroya Research Institute at Virginia Mason, Diabetes Program and Tetramer Core LaboratoryDetection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.https://doi.org/10.1038/ncomms12614
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James
spellingShingle	Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining Nature Communications
author_facet	Hannes Uchtenhagen Cliff Rims Gabriele Blahnik I-Ting Chow William W. Kwok Jane H. Buckner Eddie A. James
author_sort	Hannes Uchtenhagen
title	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_short	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_full	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_fullStr	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_full_unstemmed	Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining
title_sort	efficient ex vivo analysis of cd4+ t-cell responses using combinatorial hla class ii tetramer staining
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-08-01
description	Detection of antigen specificity in a blood sample is limited by low frequencies of individual clones. Here the authors develop an MHC class II combinatorial technique and apply it to immunophenotype human CD4+ T cells of multiple specificities in response to influenza vaccine in single blood samples.
url	https://doi.org/10.1038/ncomms12614
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Efficient ex vivo analysis of CD4+ T-cell responses using combinatorial HLA class II tetramer staining

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