Isolation and biochemical characterization of a metagenome-derived 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments

Isolation and biochemical characterization of a metagenome-derived 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase gene from subtropical marine mangrove wetland sediments

Abstract 3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase (DAHPS) is a key rate-limiting enzyme in aromatic amino acid anabolism. A new Iβ-type DAHPS gene (aro1A) was identified in a metagenomic library from subtropical marine mangrove sediment. The gene encoded a polypeptide composed of 272 ami...

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Bibliographic Details
Main Authors: Huaxian Zhao, Hua Gao, Kai Ji, Bing Yan, Quanwen Li, Shuming Mo, Minggang Zheng, Qian Ou, Bo Wu, Nan Li, Chengjian Jiang
Format: Article
Language:English
Published: SpringerOpen 2019-02-01
Series:AMB Express
Subjects:
3-Deoxy-d-arabino-heptulosonate-7-phosphate synthase
Novel gene
Metagenomic library
Subtropical marine mangrove sediment
Biochemical characterization
Online Access:http://link.springer.com/article/10.1186/s13568-019-0742-4

Internet

http://link.springer.com/article/10.1186/s13568-019-0742-4

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