Biochemical and Structural Characterization of Enolase from Chloroflexus aurantiacus: Evidence for a Thermophilic Origin

Enolase catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during both glycolysis and gluconeogenesis and is required by all three domains of life. Here, we report the purification and biochemical and structural characterization of enolase from Chloroflexus aurantiacus, a thermop...

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Bibliographic Details
Main Authors: Oleg A Zadvornyy, Eric eBoyd, Matthew ePosewitz, Nikolay A Zorin, John W. Peters
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fbioe.2015.00074/full