Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos

Summary: This protocol describes a high-throughput and multiplexed DNA fluorescence in situ hybridization method to trace chromosome conformation in Caenorhabditis elegans embryos. This approach generates single-cell and single-chromosome localization data that can be used to determine chromosome co...

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Bibliographic Details
Main Authors: Ahilya N. Sawh, Susan E. Mango
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300940